Effects of curing lights on human gingival epithelial cell proliferation.

BACKGROUND: Light-emitting diode (LED) and quartz-tungsten-halogen (QTH) curing lights are used commonly in clinics. The aim of this study was to assess the effect of these lights on the proliferation of human gingival epithelial cells. METHODS: Smulow-Glickman (S-G) cells were exposed to a VALO LED (Ultradent) or an XL3000 QTH (3M ESPE) light at 1 millimeter or 6 mm distance for 18, 39, 60, and 120 seconds. Untreated and Triton X-100 treated cells were used as controls. At 24, 48, and 72 hours after light exposure, cell proliferation was evaluated via a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. RESULTS: The authors first evaluated the performances of these 2 lights. Both LED and QTH lights generated heat. The LED light generated less heat than the QTH light and could save approximately two-thirds of the curing time. When used for 18 seconds at a 6 mm distance, the LED light did not inhibit the proliferation of S-G cells. However, if the exposure time was longer (for example, 39, 60, or 120 seconds), the LED light inhibited cell proliferation. The inhibitory effect increased when the exposure time was increased to 39, 60, or 120 seconds. The QTH light did not inhibit S-G cell proliferation if the exposure time was less than 120 seconds. CONCLUSIONS: Prolonged exposure to a blue curing light (both LED and QTH) inhibits the proliferation of gingival epithelial cells and may cause damages to oral soft tissues. PRACTICAL IMPLICATIONS: In dental practices, a balance should be struck in consideration of curing time not only to cure the composites completely but also to minimize unnecessarily prolonged light exposure.

The effect of artificial ultraviolet light on the gloss of composite resin surface sealants.

This study evaluated how exposure to ultraviolet (UV) light affected the gloss of composite resins following application of resin-based surface sealants (SSs). Thirty composite resin discs were fabricated using cylindrical molds and randomly assigned to 1 of 5 SS groups (n = 6 each). Both sides of each disc received a polyester film clear matrix surface finish, and then rotary finishing and polishing procedures were performed on 1 side only. Six baseline measurements for each disc surface were performed using a 60-degree-angle gloss meter. The finished and polished side then received an SS application and was polymerized with an LED light through a polyester film matrix. Gloss measurements were then obtained. All specimens were then exposed to an artificial UV light source for a total of 382 hours, after which final gloss measurements were made. A 2-way repeated-measures analysis of variance and Student-Newman-Keuls pairwise multiple comparison tests revealed statistically significant differences between the SS materials and surface treatments (P < 0.05). No statistically significant (P > 0.05) differences were found between the surfaces that were mechanically finished and polished and those that only received a polyester film finish. However, all specimens exhibited significantly (P < 0.0001) higher gloss readings after SS application. Following UV exposure, all SS specimens exhibited significantly (P < 0.0001) lower gloss readings. The study results suggest that application of SSs to composite resin discs increases the gloss on a short-term basis; however, long-term exposure of SSs to an artificial UV light source might have a negative effect on the surface properties. Thus, dentists need to periodically evaluate composite resin restorations.

The effect of a nanofilled resin-based coating on water absorption by teeth restored with glass ionomer.

BACKGROUND: A nanofilled, resin-based light-cured coating (G-Coat Plus, GC America, Alsip, Ill.) may reduce water absorption by glass ionomers. The authors investigated this possibility by measuring cuspal flexure caused by swelling of glass ionomer-restored teeth. METHODS: The authors cut large mesio-occlusodistal slots (4-millimeter wide, 4-mm deep) in 12 extracted premolars and restored them with a glass ionomer cement (Fuji IX GP Extra, GC America). Six teeth were coated, and the other six were uncoated controls. The authors digitized the teeth in three dimensions by using an optical scanner after preparation and restoration and during an eight-week storage in water. They calculated cuspal flexure and analyzed the results by using an analysis of variance and Student-Newman-Keuls post hoc tests (significance level .05). They used dye penetration along the interface to verify bonding. RESULTS: Inward cuspal flexure indicated restoration shrinkage. Coated restorations had significantly higher flexure (mean [standard deviation], -11.9 [3.5] micrometers) than did restorations without coating (-7.3 [1.5] µm). Flexure in both groups decreased significantly (P < .05) during water storage and, after eight weeks, it changed to expansion for uncoated control restorations. Dye penetration along the interfaces was not significant, which ruled out debonding as the cause of cuspal relaxation. CONCLUSIONS: Teeth restored with glass ionomer cement exhibited shrinkage, as seen by inward cuspal flexure. The effect of the protective coating on water absorption was evident in the slower shrinkage compensation. PRACTICAL IMPLICATIONS: The study results show that teeth restored with glass ionomers exhibited setting shrinkage that deformed tooth cusps. Water absorption compensated for the shrinkage. Although the coating may be beneficial for reducing water absorption, it also slows the shrinkage compensation rate (that is, the rate that hygroscopic expansion compensates for cuspal flexure from shrinkage).

Shrinkage stress compensation in composite-restored teeth: relaxation or hygroscopic expansion?

OBJECTIVES: Polymerization of composite restorations causes shrinkage, which deforms and thus stresses restored teeth. This shrinkage deformation, however, has been shown to decrease over time. The objective was to investigate whether this reduction was caused by hygroscopic expansion or stress relaxation of the composite/tooth complex. METHODS: Extracted molars were mounted in rigid stainless steel rings with four spherical reference areas. Twelve molars were prepared with large mesioocclusodistal slots, etched, bonded, and restored with a composite material (Filtek Supreme, 3M ESPE) in two horizontal layers. Ten intact molars were the controls. The teeth were stored either in deionized water or silicone oil. They were scanned after preparation (baseline), restoration (0-week), and after 1, 2, and 4 weeks storage. Scanned tooth surfaces were aligned with the baseline using the unchanged reference areas. Cuspal flexure was calculated from lingual and buccal surface deformation. To verify that the restorations had remained bonded, dye penetration at the interfaces was assessed using basic fuchsin dye. Statistical assessment was done by ANOVA followed by Student-Newman-Keuls post hoc test (p=0.05). RESULTS: Substantial cuspal contraction was found for restored teeth after the composite was cured (13-14 µm cuspal flexure). After 4 weeks cuspal contraction decreased significantly for restored teeth stored in water (7.3 ± 3.2) but not for those stored in silicone oil (11.4 ± 5.0). Dye penetration of the occlusal interface was minimal in both groups (106 ± 87 and 21 ± 28 µm in water and silicone oil, respectively). SIGNIFICANCE: The results suggest that hygroscopic expansion was the main mechanism for shrinkage stress compensation.